Optical alignment device for two-photon microscopy
نویسندگان
چکیده
منابع مشابه
Combined two-photon microscopy and angiographic optical coherence tomography.
A combined two-photon microscopy (TPM) and angiographic optical coherence tomography (OCT) is developed, which can provide molecular, cellular, structural, and vascular information of tissue specimens in vivo. This combined system is implemented by adding an OCT vasculature visualization method to the previous combined TPM and OCT, and then is applied to in vivo tissue imaging. Two animal model...
متن کاملContinuous wave two-photon scanning near-field optical microscopy.
We have implemented continuous-wave two-photon excitation of near-UV absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 647-nm emission of an Ar-Kr mixed gas laser was used to excite the UV-absorbing DNA dyes DAPI, the bisbenzimidazole Hoechst 33342, and ethidium bromide in a shared aperture SNOM with uncoated fiber tips. Polytene chromosomes of Drosophila melanogast...
متن کاملAdaptive optical two-photon microscopy using autofluorescent guide stars.
We demonstrate a fast, direct wavefront-sensing method for dynamic in vivo adaptive optical two-photon microscopy. By using a Shack-Hartmann wavefront sensor and open-loop control, the system provides high-speed wavefront measurement and correction. To measure the wavefront in the middle of a Drosophila embryo at early stages, autofluorescence from endogenous fluorophores in the yolk were used ...
متن کاملA New Phase for Two-Photon Microscopy
polarization, also participates in the LTS in TC cells, particularly during sleep spindles. The effects of its distribution within the TC cell could also be studied with compartmental models, in conjunction with the distribution of the T-currents. The optimal distributions of these currents may not be independent of each other, so they need to be jointly varied. The calcium that enters the neur...
متن کاملFast Scanning Two-Photon Microscopy
Fast scanning two-photon microscopy coupled with the use light activated ion channels provides the basis for fast imaging and stimulation in the characterization of in vivo neural networks. A two-photon microscope capable of fast scanning using acousto-optic deflectors was designed and implemented. The software controller was expanded so that random access scan in three dimensions could be hand...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2018
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.9.003624